Regulation of ZAP-70 Intracellular Localization: Visualization with the Green Fluorescent Protein

Regulation of ZAP-70 Intracellular Localization: Visualization with the Green Fluorescent Protein

To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelial cells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the plasma membrane upon cellular activation, a phenotype enhanced by the coexpression o...

Mapping intracellular temperature using green fluorescent protein.

Heat is of fundamental importance in many cellular processes such as cell metabolism, cell division and gene expression. (1-3) Accurate and noninvasive monitoring of temperature changes in individual cells could thus help clarify intricate cellular processes and develop new applications in biology and medicine. Here we report the use of green fluorescent proteins (GFP) as thermal nanoprobes sui...

Intracellular Localization and Transcriptional Activation by Glucocorticoid Receptor - Green Fluorescent Protein ( GFP ) Fusion Proteins the Human

of acting as an intracellular signal transducer [3, 4, 13]. It has long been known that in the absence of its hormonal ligands, GR localizes in the cytoplasm forming a docking complex with heat shock proteins such as Hsp90, Hsp70, and an immunophilin [12], Upon binding to ligand, GR dissociates from the cytoplasmic docking complex and rapidly translocates into the nucleus dependent on its nucle...

Simultaneous visualization of the translocation of protein kinase Calpha-green fluorescent protein hybrids and intracellular calcium concentrations.

The alpha isoform of protein kinase C (PKCalpha) is a ubiquitous protein kinase, which, upon activation, translocates rapidly from the cytoplasm to the plasma membrane. To follow this translocation, PKCalpha was tagged with a highly fluorescent derivative of green fluorescent protein and stably expressed in baby hamster kidney cells overexpressing the muscarinic type 1 receptor. Addition of the...

Zap - 70 ( zeta - chain - associated protein 70 kD ) deficiency